Date of Award

1970

Embargo Period

8-1-2024

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Microbiology and Immunology

College

College of Graduate Studies

First Advisor

Norman L. Goodman

Abstract

Commercially available reagent-impregnated paper strip tests and tablet substrate tests were evaluated in comparison to the standard biochemical tests using representative members of the Enterobacteriaceae. For the most part, these tests were found to be unreliable or difficult to interpret. Standard media for key biochemical tests were then modified and a new inoculation procedure developed to allow for test results after four hours incubation. Representative members of the Enterobacteriaceae were tested using the modified standard media and, where a given test was critical for the differentiation of the organisms, the rapid test results were in complete agreement with the control test results. The same Enterobacteriaceae were tested to determine their ability to tolerate varying concentrations of sodium chloride. The pathogenic species were more sensitive to this salt than were the common saprophytes. The former were inhibited at concentrations exceeding four per cent while the latter tolerated six to eight per cent sodium chloride.

Rights

All rights reserved. Copyright is held by the author.

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