Date of Award

1-1-1975

Embargo Period

5-10-2024

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Basic and Clinical Immunology and Microbiology

College

College of Graduate Studies

First Advisor

Thomas A. Mahvi

Second Advisor

John B. Hynes

Third Advisor

C. D. Graber

Fourth Advisor

T. Hagood

Abstract

The effect of varying concentrations of 2,4-Diamino-6-(3,4-Dichlorophenylacetamido) quinazoline (DDPQ) on viable cells of cryptococcus neoformans at 37 C reveals that the compound is fungistatic at concentrations in the range of 1 to 6 ug/ml and fungicidal at concentrations above 6 ug/ml. The effect of DDPQ on C. neoformans can be measured within 30 minutes after incubation with DDPQ. Manometric studies show a decrease in oxygen uptake in both exogenous and endogenous flasks after 120 minutes. Flame spectrophotometry shows no ion leakage of sodium or potassium which suggests that no cell membrane damage is involved. Incorporation studies show an almost immediate inhibition in uptake of labeled uridine, while leucine uptake is inhibited after 20 minutes exposure to DDPQ. This suggests a primary block in RNA synthesis, secondarily affecting protein synthesis.

Rights

Copyright is held by the author. All rights reserved.

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