Date of Award
1995
Document Type
Thesis
Degree Name
Master of Biomedical Science
College
College of Graduate Studies
First Advisor
Lisa Matsuda
Abstract
The cannabinoid receptor gene has recently been cloned. Comparisons of cDNA clones with genomic DNA show that the gene consists of a coding region and at least two untranslated 5' exons (A and B). Two alternative transcripts, differing in their 5' untranslated sequence, have been found that code for identical receptors. Primer extension for transcript A reveal that its most 5' transcriptional start site corresponds to nucleotide -548. Additional transcripts initiated 3' of -548 may exist. Ribonuclease protection assay and primer extension for transcript B reveal that its most 5' transcriptional start site corresponds to nucleotide -406. It is possible that shorter transcripts, corresponding to transcriptional start sites 3' of -406 may exist.
Recommended Citation
Baden, Jordana, "Mapping the Transcriptional Start Site(s) of the Cannabinoid Receptor Gene" (1995). MUSC Theses and Dissertations. 69.
https://medica-musc.researchcommons.org/theses/69
Rights
All rights reserved. Copyright is held by the author.