Date of Award

1976

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Microbiology and Immunology

College

College of Graduate Studies

First Advisor

John P. Manos

Second Advisor

N. M. Burdash

Abstract

Macrophage migration inhibition factor (MIF) is a lymphocyte-derived substance which plays an important role in cell mediated immunity. Soluble factors containing MIF-like activity and produced by non-stimulated and virus-infected non-lymphoid cells have also been reported. In the present study, a MIF-like factor was repeatedly detected in Buffalo green monkey kidney cells infected with mumps or herpes simplex virus (HSV) indicating that this substance is reproducible and can be stimulated by a RNA and a DNA virus. Wistar-38 (WI-38) cell cultures also increased production of this substance in response to mumps but not HSV infection, indicating that the production of this factor is not limited to one cell line, and is not necessarily induced by all viruses. A factor which stimulated the spread of macrophages was also found to be induced in WI-38 cells by both viruses, suggesting another possible defense mechanism by non-lymphoid cells in response to viral infection. The ability of non-stimulated WI-38 cells to produce MIF-like activity was also confirmed, and this factor could be further stimulated or opposed by viral infection.

Rights

All rights reserved. Copyright is held by the author.

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