Date of Award

2016

Embargo Period

8-1-2024

Document Type

Thesis

Degree Name

Master of Science in Dentistry

College

College of Dental Medicine

First Advisor

Timothy Rohde

Second Advisor

Joseph Assad

Third Advisor

Robert P. Bethea

Abstract

Introduction: The aim of the study was to directly assess the depth of sealer penetration into dentinal tubules following different final rinses and indirectly evaluate precipitation of irrigating solutions. Materials and Methods: 52 extracted maxillary incisors were prepared to size 40/04 crown down with 6% sodium hypochlorite irrigating solution. Teeth were randomly divided into 4 groups (n=13) according to the final rinse. Group 1- 17% EDTA, saline and 2% Chlorhexidine solution; Group 2- SmearOFF™; Group 3- QMix®; Group 4- control with saline. Each final rinse was 3 ml over 1 minute placed with a 30-gauge needle 3 mm short from working length. Obturation with gutta percha and resin sealer mixed with rhodamine B isothiocyanate fluorescent dye using warm vertical compaction. Teeth were sectioned into apical, middle and coronal thirds using Buehler diamond saw at 3, 6 and 9 mm from the apex. Sections were examined under confocal laser scanning microscopy and images were exported to ImageJ software to measure the maximum depth of sealer penetration circumferentially. Results: Data were analyzed using a generalized linear model and Post hoc multiple comparisons with significance set at P < .05. Group 3 had the highest mean depth of penetration. There were statistically significant differences in sealer penetration between Group 2 and the control for all sections (apical, middle, and coronal), between Group 3 and the control for all sections, and, lastly, between Group 1 and the control in the apical section only. Conclusions: Recently developed endodontic final rinses SmearOFF™ and QMix® produced more patent dentinal tubules and better sealer penetration than using EDTA and chlorhexidine sequentially.

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