Document Type

Article

Embargo Period

12-1-1977

Publication Date

12-1-1977

Abstract

It has been shown that macrophage and granulocyte colonies can be induced m semisolid agar (1-3) in the presence of substances termed colony-stimulating factors (CSF), which are released predominantly by monocytes (4). However, attempts to induce formation of lymphoid colonies with CSF have so far proved unsuccessful. In the mouse, B lymphoid colonies are formed in the presence of 2-mercaptoethanol (5), and T lymphoid colonies can be induced with the plant lectins phytohemagglutinin (PHA) and concanavalin A (6). T lymphoid colonies can also be established from human peripheral blood lymphocytes in the presence of PHA (7-9), whereas with pokeweed mitogen mixed T and B lymphoid colonies are formed (9). Established human lymphoid cell lines multiply spontaneously in the absence of plant lectins or mercaptoethanol, and it seemed possible that such cells might release growth-stimulating substances into the culture medium. We have therefore investigated the ability of conditioned medium (CM) obtained from lymphoid cell lines to induce normal human peripheral blood lymphocytes (PBL) to form lymphoid colonies in agar.

Journal

Journal of Experimental Medicine

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