Homologies between T Cell Receptor Junctional Sequences Unique to Multiple Sclerosis and T Cells Mediating Experimental Allergic Encephalomyelitis

Authors

Mark Allegretta, Stanford University School of Medicine
Richard J. Albertini, University of Vermont
Mark D. Howell
Lawrence R. Smith
Roland Martin, National Institutes of Health
Henry F. McFarland, National Institutes of Health
Subramamiam Sriram, University of Vermont
Steven Brostoff, Medical University of South Carolina
Lawrence Steinman, Stanford University School of Medicine

Document Type

Article

Embargo Period

7-1-1994

Publication Date

7-1-1994

Abstract

The selection of T cell clones with mutations in the hypoxanthine guanine phosphoribosyltransferase (hprt) gene has been used to isolate T cells reactive to myelin basic protein (MBP) in patients with multiple sclerosis (MS). These T cell clones are activated in vivo, and are not found in healthy individuals. The third complementarity determining regions (CDR3) of the T cell receptor (TCR) α and β chains are the putative contact sites for peptide fragments of MBP bound in the groove of the HLA molecule. The TCR V gene usage and CDR3s of these MBP-reactive hprt- T cell clones are homologous to TCRs from other T cells relevant to MS, including T cells causing experimental allergic encephalomyelitis (EAE) and T cells found in brain lesions and in the cerebrospinal fluid (CSF) of MS patients. In vivo activated MBP-reactive T cells in MS patients may be critical in the pathogenesis of MS.

Journal

Journal of Clinical Investigation

Recommended Citation

Allegretta, Mark; Albertini, Richard J.; Howell, Mark D.; Smith, Lawrence R.; Martin, Roland; McFarland, Henry F.; Sriram, Subramamiam; Brostoff, Steven; and Steinman, Lawrence, "Homologies between T Cell Receptor Junctional Sequences Unique to Multiple Sclerosis and T Cells Mediating Experimental Allergic Encephalomyelitis" (1994). MUSC Faculty Journal Articles. 101.
https://medica-musc.researchcommons.org/facarticles/101