Date of Award
Master of Science (MS)
College of Graduate Studies
Amy D. Bradshaw
Heart failure is one of the leading causes of death in the United States. Heart failure with preserved ejection fraction (HFpEF) is commonly associated with individuals with hypertension. Increases in myocardial hemodynamic load are recapitulated in murine models of pressure overload (PO) induced by trans-aortic constriction (TAC). In both human and TAC hearts, increases in fibrosis are demonstrated and align with increases in myocardial stiffness, a hallmark of diastolic dysfunction in HFpEF. Previous studies indicated that the matricellular protein secreted protein acidic and rich in cysteine (SPARC) was essential for the development of cardiac fibrosis in TAC hearts, however the mechanisms behind the functional significance of SPARC in cardiac fibrosis has yet to be defined. This study utilizes a bone marrow transfer model to determine the role of SPARC expression in bone-marrow derived cells during the onset of cardiac fibrosis induced by TAC. Following induction of PO by TAC, SPARC-null mice transplanted with WT bone marrow demonstrated levels of fibrosis similar to that of wild type hearts despite the abrogation of SPARC expression by resident cardiac cells. Likewise, WT mice transplanted with SPARC null bone marrow developed very little fibrosis despite SPARC expression by resident cardiac cells. Interestingly, SPARC-null mice with WT bone marrow showed an increase in the cardiac macrophage population as well as increased levels of VCAM-1 expression at two weeks post TAC versus WT mice with SPARC-null bone marrow. In conclusion, SPARC-dependent macrophage recruitment to the myocardium is a significant factor to the development of fibrosis after TAC and may offer a novel target for future strategies in the treatment of HFpEF.
Riley, Hannah Jeanne, "SPARC Produced by Bone-Marrow Derived Cells Contributes to Myocardial Fibrosis" (2019). MUSC Theses and Dissertations. 230.
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